- 询价
- GeneTex
- 美国
- GTX16373
- 2025年07月10日
- WB, IP, ELISA
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store Buffer A and Buffer B at -20ºC, and the rest of the kit at room temperature.
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX16373
- 级别：
Research Kits
- 库存：
Available
- 供应商：
GeneTex
- 应用范围：
WB, IP, ELISA
- 靶点：
Trident Membrane Protein Extraction Kit
- 抗体英文名：
Trident Membrane Protein Extraction Kit
- 抗体名：
Trident Membrane Protein Extraction Kit
- 规格：
20 test, 5 test
HepG2 cells were lysed and extracted by GTX16373 Plasma Membrane Protein Extraction kit.
When following the protocol, cell extracts would be isolated into cytosolic and total membrane.
The fractions were then assayed by Western Blot analysis, membrane was blotted with a plasma membrane protein- Na/K ATPase antibody (subcellular marker), a nucleolar small nuclear ribonucleoprotein- Fibrillarin antibody (GTX113684, subcellular marker) and beta Catenin antibody (GTX101435, internal control).
Abbreviations:
WCE : whole cell extract
CE : cytosolic extract
ME: total membrane extract
NE: nuclear extract
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文献和实验Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
The plasma membrane (PM) exists as the interface between the cytosol and the environment in all living cells and is one of the most complex and differentiated membrane. The identification and characterization of membrane proteins
A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible
Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) was updated to meet current needs in the field of proteomics. This protocol summarizes our experience using this method since its introduction over 30 years ago
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