- 询价
- GeneTex
- 美国
- GTX04142-06
- 2025年07月12日
- IHC-Fr, FACS, ELISA
- Mouse
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rat IgG2c hybridoma
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
Purified IgG2a
- 适应物种：
Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 目录编号：
GTX04142-06
- 级别：
Secondary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
IHC-Fr, FACS, ELISA
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Mouse Anti-Rat IgG2c
- 抗体英文名：
Mouse Anti-Rat IgG2c antibody [2C8F1] (FITC)
- 抗体名：
Mouse Anti-Rat IgG2c 抗体 [2C8F1] (FITC)
- 规格：
500 μg
ELISA analysis of purified rat immunoglobulins using serially diluted GTX04142-06 Mouse Anti-Rat IgG2c antibody [2C8F1] (FITC).
FACS analysis of BALB/c mouse splenocytes using Rat Anti-Mouse CD24 antibody followed by GTX04142-06 Mouse Anti-Rat IgG2c antibody [2C8F1] (FITC).
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文献和实验1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
。 ④其他试剂和材料 l％叠氮化钠、离心机及离心管、烧杯(25m1)、搅拌器、无菌吸管及毛细滴管、烧杯(500m1)透析袋等。 (2)方法及步骤 ①取高效价的抗人球蛋白兔免疫血清，分离球蛋白，用盐水(0．15mol／LNaCl)及缓冲液(0．5mol／LNaHC03—Na2C03，pH9．0)稀释使每毫升内含蛋白质lOmg，缓冲液为总量的10％，降温至4℃。 ②加入异硫氰酸盐(FITC)荧光素[蛋白：荧光素＝(50—80)mg‘lmg]，在0～4~C下电磁搅拌12～
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