Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau
宿主
Mouse
反应种属
Human, Mouse, Rat
应用
WB: 1:1000, IHC-P: 1:2000
性能
形式
Liquid
浓度
2 mg/Ml
纯化方法
Protein G affinity column
类型
Monoclonal Antibody
克隆号
R145
储存/保存方法
Store at -20℃ for one year.
存储溶液
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明
Tau is a microtubule-associated protein for which the physiological functions remain a topic of vigorous investigation. Additionally, tau is a central player in the pathogenesis of several diseases such as Alzheimer’s disease and several frontotemporal dementias. A critical variable to understanding tau in physiological and disease contexts is its normal localization within cells of the adult CNS.
细胞定位
Cell Membrane, Secreted, Cytoplasm
实验结果图:
WB result of Tau mouse mAb Primary antibody: Tau mouse mAb at 1/1000 dilution Lane 1: mouse liver lysate 20ug Lane 2: mouse brain lysate 20ug Negative control: mouse liver lysate Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50~65 kDa Observed MW: 50~65 kDa Exposure time: 90s
IHC shows positive staining in paraffin-embedded human colon. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.