宿主细胞蛋白（大肠杆菌）ELISA试剂盒|Host Cell

宿主细胞蛋白（大肠杆菌）ELISA试剂盒，Host Cell Proteins (E.coli) ELISA kit，宿主细胞蛋白（大肠杆菌）ELISA试剂盒,Host Cell Proteins (E.coli) ELISA kit

产品名称：宿主细胞蛋白（大肠杆菌）ELISA试剂盒-Host Cell Proteins (E.coli) ELISA kit

产品货号：A05034

产品规格：96wells

背景资料：Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as Host Cell Proteins (HCP) are generated and need to be removed. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or research bioproducts.|Polyclonal antibodies used in this kit have been generated against several strains of E.coli and specifically selected for their recognition of a large spectrum of E. coli proteins. Thus, this kit can be considered as generic and allows a relative-quantitative determination of E. coli HCP in many types of samples, such as samples issued from the purification process (HCP clearance), process control, quality control, or product release.|Using this kit, HCP concentration is measured in ng/ml (HCP equivalent is extrapolated from a standard curve). Conventionally, the HCP content in a product will finally be expressed in ng/mg, where ng represents HCP mass and mg represents the product mass. Note that, contrary to the concentration measurement of the product, the HCP signal is only reflective of antibody binding and does not strictly reflect the mass of HCP.|This kit has been successfully validated for recovery and precision using reconstituted HCP samples and tested against different final products. Given the diversity of final products, all potential matrix effects cannot be known and it is recommended that you test the suitability of the kit for use with your own HCP samples in your laboratory.|This kit should be used as one part of your complete HCP analysis.

保存建议：宿主细胞蛋白（大肠杆菌）ELISA试剂盒-Host Cell Proteins (E.coli) ELISA kitStore at +4degrees; shelf life 6 months maximum after production

产品描述：酶免疫测定（EIA）是一种检测和定量样品中抗原（蛋白质、激素等）或抗体的技术。它依赖于抗体结合特定抗原的能力。用酶标记抗体或抗原，所述酶的底物是转化成可测量产物（颜色或荧光）的色原或荧光团。|酶联免疫吸附测定（ELISA）是使用固相（例如：微量滴定板）上包被有固定分子的抗原以进行检测。随着时间的推移，科学家们已经将术语ELISA扩展到使用抗体包被固相的EIA。这就解释了为什么我们使用包被抗体的EIA试剂盒也被称为ELISA试剂盒。|杂质评估是重组蛋白（包括治疗性蛋白）的药物开发生产过程中的关键步骤。来自介导蛋白质表达的细胞的特定杂质（称为宿主细胞蛋白质（HCP））被产生并且需要被去除。该试剂盒预期用于评估大肠杆菌的相对量。生产或研究生物制品中的大肠杆菌HCP。|该试剂盒中使用的多克隆抗体已经针对几种大肠杆菌菌株产生，并且针对它们识别大范围的大肠杆菌而被特异性地选择。coli蛋白。因此，该试剂盒可被认为是通用的，并允许相对定量测定E。在许多类型的样品中，例如从纯化过程（HCP清除）、过程控制、质量控制或产品放行中发出的样品，可以检测到大肠杆菌HCP。|使用该试剂盒，以ng/ml测量HCP浓度（HCP当量从标准曲线外推）。通常，产品中的HCP含量最终以ng/mg表示，其中ng表示HCP质量，mg表示产品质量。请注意，与产品的浓度测量相反，HCP信号仅反映抗体结合，并不严格反映HCP的质量。|已使用复溶HCP样品成功验证了该试剂盒的回收率和精密度，并针对不同的最终产品进行了检测。鉴于最终产品的多样性，无法了解所有潜在的基质效应，建议您在实验室检测试剂盒与您自己的HCP样本一起使用的适用性。|该试剂盒应作为完整HCP分析的一部分使用。

点击：宿主细胞蛋白（大肠杆菌）ELISA试剂盒-Host Cell Proteins (E.coli) ELISA kit查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询艾美捷科技有限公司。更多/Bertin-Bioreagent公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。

法国Bertin Technologies公司是一家大型仪器设备及试剂供应商，拥有50多年的历史，产品涉及卫生、环境、安全甚至军工等诸多领域。Bertin Bioreagent是Bertin Technologies集团的生物试剂部门，主要是致力于研发与炎症，氧化损伤，内分泌学，糖尿病，肥胖症，高血压，精神药理学，癌症，哮喘，朊病毒疾病，药代动力学和代谢等相关的化学试剂 ，蛋白，底物，抗体及检测试剂盒等产品。 Bertin Instruments是Bertin Technologies集团的仪器部门，其中旗舰产品Precellys系列多功能生物样品匀浆器是全世界各个实验室的明星产品，截至目前累计有15000+的引文！

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