In Vitro: Antileishmanial agent-16 (0-20 µM; 24 h) shows superior activity to againsts L. Major promastigote and amastigote forms, with IC50s of 0.59 and 0.81 µM, respectively. Antileishmanial agent-16 shows high selectivity towards L. amastigote as well as their safety on mammalian cells (VERO cells), with anSI value of 311.4. Selectivity indice (SI): the ratio between the in vitro cytotoxic activity (CC50 in μM) and the antileishmanial activity against amastigote form (unit of IC50: μM).