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文献和实验the loop remove the cap from the culture and gently flame the top of the test tube (do not overheat). Insert the flamed inoculating loop into the test tube until it is submersed in the broth. The loop should be allowed to cool slightly before immersion
the collection rack with clean 1.5 ml screw-capped tubes into the manifold. Clamp the manifold with the white clamps, and apply 300 ul of 10:1 TE buffer heated to 65degC and pull the eluted DNA through at 5 in. Hg. After the liquid has filtered through, raise
the collection rack with clean 1.5 ml screw-capped tubes into the manifold. Clamp the manifold with the white clamps, and apply 300 ul of 10:1 TE buffer heated to 65degC and pull the eluted DNA through at 5 in. Hg. After the liquid has filtered through, raise
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