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文献和实验Boric Acid 0.02M Disodium EDTA ) (Advanced Biotechnologies)Acrylease (Stratagene)Glass cleaner (e.g., Windex, Glass Plus)95% ethanol2. EquipmentThermal cycler (MJ Research)Sequencing gel electrophoresis apparatus (Gibco BRL)High voltage power supplyGel
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue
Introduction In vivo gene delivery is gaining increasing momentum for gene therapy as delivery methods become more advanced and safe. This raises the need for precise, sensitive, and easy evaluation of gene transfection efficacy. A method
Northern Blotting方法(Howell Lab)
bottom twice more with 100 µl buffer each time. Add 750 µl 100% EtOH and let precipitate overnight at -70℃. Spin down at 4℃ for 12 min, dry in the TC hood, and resuspend with DEPC water. RNA quantitation Let resuspended RNA sit on ice
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