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文献和实验Lumigen HyPerBlu provides sensitive chemiluminescent detection of hydrogen peroxide
it an excellent choice for examination of oxidase activity. The signal intensity is sufficient for measurement on PMT based plate reading luminometers or CCD imaging systems. HyPerBlu technology detection is suitable for automation and miniaturization to 384
may be grown to attain exponential growth before adding the IPTG. Add 10 - 100 µl of 1 M IPTG so that the final concentration of IPTG is from 1 - 10 mM. Induce protein synthesis for 1 - 3 hours. The final yield of protein may be affected by the concentration
Preparation of Nested Deletions
, then add 10 µl of 3 M sodium acetate, pH 5.2, and 200 µl of 100% ethanol. Spin for 15 min at 4℃. Remove supernatant. Add 500 µl of 70% ethanol, vortex gently, then spin for 5 min at 4℃. Remove supernatant, add more 70% ethanol, vortex, spin, pull of ethanol
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