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文献和实验96 Well Mating Protocol platewise
is between the H1H2 recombination regions which lie just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation: 1. Streak fresh donor plasmid on LB proper antibiotic IPTG/XGAL plate (donor F’ strain contains lac , it will be blue
96 Well Mating Protocol platewise
just inside two I-SceI cut sites (Sce1-H1-Cassette- 96 well Mating Protocol Preparation:1. Streak fresh donor plasmid on LB + proper antibiotic +IPTG/XGAL plate (donor F’ strain contains lac +, it will be blue on the XGAL plate2. Streak recipient plasmid (pMSCV) on LB
Kingfisher Flex 96 Plant DNA High Pure Protocol
for pipette 7. Ice Bucket 8. 1.5 or 2.0 mL microcentrifuge tubes, sealed deep-well plate or capped microtube rack for sample disruption 9. Kingfisher 96 or Kingfisher Flex 96 with Deep Well Magnet 10. 4 Deep Well Kingfisher 96 Plates
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