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文献和实验Preparation of a DNA Sequencing Gel
the sharkstooth comb so that the teeth rest firmly on the gel, but are not digging into the top of the gel. 4) Load the gel (1.2 - 1.5 uL per lane) by placing the end of the pipet tip in the space between the glass plates, directly over the well. Dispense
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
1. Excision of protein bands (spots) from polyacrylamide gels Rinse the gloves you use with water to avoid traces of dust in your sample. Rinse the gel with water. Excise spots with clean pipette tip (f 2 mm) cutting
gel has set, place in gel rig and immerse in buffer. 7. Prior to running the gel, flush the wells out thoroughly with running buffer. D. Running the gel 1. After flash spinning the samples, load into the wells. 2. Be sure to use markers. We use
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