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文献和实验2-D Polyacrylamide Gel Electrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives.Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study. Solutions TIP
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20ul TRYPSIN/EDTA. Check using microscope that there are single colonies
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
and break up. Add a small volume of PBS to plate, hold plate over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20
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