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文献和实验of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap
and resuspended in protein-free medium or salt solution prior to counting.Supplies & Equipment:Eppendorf tubes (1.5 ml)Micropipet (10µl)HemocytometerReagents:HBSS (Hanks' Balanced Salt Solution)sterile Trypan blue solution 0.4% (Sigma T-8154)Procedure:Prepare
(Hanks' Balanced Salt Solution) sterile Trypan blue solution 0.4% (Sigma T-8154) Procedure: Prepare a cell suspension in HBSS Transfer into Eppendorf tube: 0.5 ml of 0.4% Trypan blue solution 0.3 ml
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