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文献和实验RNAase Dissolve in 50 mM KAC pH 5.5 Heat to 100 o C for 15 minutes and cool slowly to room temperature Store at -20. (common freezer for stock solutions) 上一篇
Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate
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