Pepsinogen (PG) is the precursor of pepsin and is composed of five hypotypes: pepsinogen A (PGA), pepsinogen B (PGB), pepsinogen C (PGC), pepsinogen F (PGF) and chymosinogen [PMID: 28546787]. Pepsinogen C, also known as progastricsin or PG II, is an aspartic protease expressed primarily in gastric chief cells [PMID: 14578117].
细胞定位
Secreted
实验结果图:
WB result of PG II/PGC Rabbit mAb Primary antibody: PG II/PGC Rabbit mAb at 1/2500 dilution Lane 1: mouse liver lysate 20 µg Lane 2: mouse stomach lysate 20 µg Negative control: mouse liver lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 42 kDa Observed MW: 38 kDa Exposure time: 20s
IHC shows positive staining in paraffin-embedded human stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.