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- 英文名:
AAT Bioquest22900 Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*200Tests
- 供应商:
北京云肽生物科技有限公司
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。AAT Bioquest会不断介绍新产品,快速的丰富各个领域的产品。
我们北京云肽生物科技有限公司作为AAT Bioquest公司的特约经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
AAT Bioquest22900 Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*200Tests,产品简介:
产品品牌:AAT Bioquest
产品货号:22900
产品名称:Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*
产品规格:200Tests
产品说明:
活性氧(ROS)是氧正常代谢的天然副产物,在细胞信号传导中起重要作用。然而,在氧化应激相关状态下,ROS水平会急剧增加。ROS的积累导致细胞结构的严重损害。氧化应激在心血管疾病,糖尿病,骨质疏松症,中风,炎症性疾病,许多神经退行性疾病和癌症中的作用已经得到很好的确立。ROS测量将有助于确定氧化应激如何调节不同的细胞内途径。细胞计™荧光 ROS 检测试剂盒使用我们独特的 ROS 传感器来量化活细胞中的 ROS。ROS绿色是细胞渗透性的。当它与ROS反应时,它会产生绿色荧光。该试剂盒是一种优化的“混合和读取”检测形式,与 HTS 液体处理仪器兼容。细胞计™荧光 ROS 检测试剂盒提供灵敏的一步荧光检测,可在孵育一小时后检测活细胞中的细胞内 ROS。该测定可以以方便的 96 孔或 384 孔微量滴定板形式进行,无需分离步骤即可轻松适应自动化。其信号可以使用荧光酶标仪或荧光显微镜轻松读取。
组件
组件 A:Amplite™ ROS 绿色 1瓶
组分B:检测缓冲液 1 瓶 (20 mL)
组件C:DMSO 1 瓶 (200 μL)
示例协议
一目了然
协议摘要
- 在生长培养基中制备细胞
- 添加 Amplite™ ROS Green 工作溶液(100 孔板为 96 μL/孔,25 孔板为 384 μL/孔)
- 将细胞在 37°C 下染色 60 分钟
- 用测试化合物处理细胞以诱导ROS
- 监测Ex/Em= 490/525 nm(截止= 515 nm)下的荧光增加(底部读数模式)或使用FITC滤光片组的荧光显微镜
重要事项 在开始实验之前,在室温下解冻所有组分。
储备液的制备
除非另有说明,否则所有未使用的储备溶液应分成一次性等分试样,并在制备后储存在-20°C。避免反复冻融循环。
- Amplite ROS 绿色储备溶液 (500X):将 40 μL DMSO(组分 C)加入小瓶 Amplite ROS 绿色储备溶液(组分 A)中,充分混合制成 500X Amplite™ ROS 绿色储备溶液。
工作溶液的制备
将 20 μL 500X Amplite ROS Green 储备溶液加入 10 mL 检测缓冲液(组分 B)中,充分混合制成 Amplite™™ ROS Green 工作溶液。注意:这种Amplite™ ROS绿色工作溶液在室温下至少可稳定保存2小时。
实验方案样本
- 将 100 μL/孔(96 孔板)或 25 μL/孔(384 孔板)的 Amplite™ ROS Green 工作溶液添加到细胞板中。
- 将细胞在 5% CO 中孵育2,37°C培养箱一小时。
- 在所需的缓冲液(如PBS或HHBS)中用20 μL 11X测试化合物(96孔板)或10 μL 6X测试化合物(384孔板)处理细胞。对于对照孔(未处理的细胞),加入相应量的复合缓冲液。
- 为了诱导ROS,在室温或5%CO中孵育细胞板2,37°C培养箱至少15分钟或所需时间(用30mM H处理的Hela细胞为1分钟2O2).
- 使用荧光酶标仪(底部读取模式)在Ex / Em = 490 / 525 nm(截止= 515 nm)下监测荧光增加,或使用带有FITC滤光片组的荧光显微镜观察细胞。
产品订购信息:
| 品牌 | 货号 | 产品名称 | 规格 |
| AAT Bioquest | 22900 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence* | 200 Tests |
| AAT Bioquest | 22901 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence* | 200 Tests |
以下是AAT Bioquest品牌部分产品订购信息,如需其他产品请联系本店客服
| 品牌 | 货号 | 产品名称 | 单位 |
| AAT Bioquest | 22622 | Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence* | 200 Tests |
| AAT Bioquest | 22623 | Cell Explorer™ Live Cell Tracking Kit *Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22624 | Cell Explorer™ Live Cell Tracking Kit *Deep Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22635 | Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence* | 100 Tests |
| AAT Bioquest | 22636 | Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence* | 100 Tests |
| AAT Bioquest | 22640 | Cell Navigator®CDy6 Mitosis Imaging Kit | 100 Tests |
| AAT Bioquest | 22656 | Cell Navigator® Lysosome Staining Kit *Green Fluorescence* | 500 Tests |
| AAT Bioquest | 22670 | Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence* | 500 Assays |
| AAT Bioquest | 22674 | MitoLite™ Blue FX490 | 500 Tests |
| AAT Bioquest | 22675 | MitoLite™ Green EX488 | 500 Tests |
| AAT Bioquest | 22676 | MitoLite™ Orange FX570 | 500 Tests |
| AAT Bioquest | 22677 | MitoLite™ Red FX600 | 500 Tests |
| AAT Bioquest | 22789 | Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence* | 200 Tests |
| AAT Bioquest | 22800 | Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays* | 500 Tests |
| AAT Bioquest | 22801 | Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* | 100 Tests |
| AAT Bioquest | 22802 | Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22804 | Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22809 | Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation* | 100 Tests |
| AAT Bioquest | 22823 | Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22824 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22828 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* | 100 Tests |
| AAT Bioquest | 22829 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* | 100 Tests |
| AAT Bioquest | 22837 | Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22838 | Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22839 | Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22840 | Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors* | 100 Tests |
| AAT Bioquest | 22841 | Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22842 | Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22860 | Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100 tests |
| AAT Bioquest | 22900 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence* | 200 Tests |
| AAT Bioquest | 22901 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22903 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22904 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry* | 100 Tests |
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文献和实验, the intracellular ROS level was determined using flow cytometry.2.10. Phagocytic uptakeTo measure the phagocytic activity of RAW264.7 cells, a previously reported method was used with slight modifications [17]. RAW 264.7 cells (1 × 106 cells/mL) were preincubated
Appendix H: Radioactive tracers
to protect the user. The half-life determines both the remaining activity after storage or use, and the time that the isotope must be stored before disposal. In cell biology, only a few of the many radioactive elements are used routinely
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
, cell-cycle stage and intracellular metabolic state. The key function of mitochondria is energy production through oxidative phosphorylation (OxPhos) and lipid oxidation.1,2 Several other metabolic functions are performed by mitochondria, including urea
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