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99
- 英文名:
AAT Bioquest22809 Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation* 100Tests
- 供应商:
北京云肽生物科技有限公司
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色),荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。AAT Bioquest会不断介绍新产品,快速的丰富各个领域的产品。
我们北京云肽生物科技有限公司作为AAT Bioquest公司的特约经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
AAT Bioquest22809 Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation* 100Tests,产品简介:
产品品牌:AAT Bioquest
产品货号:22809
产品名称:Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*
产品规格:100Tests
产品说明:
| 激发(纳米) 415 |
发射(纳米) 499 |
有多种参数可用于监测细胞凋亡。该特定试剂盒旨在通过测量还原型谷胱甘肽(GSH)的降低来检测细胞凋亡。谷胱甘肽对于维持细胞的氧化还原水平很重要。它参与许多细胞过程,包括清除自由基、药物解毒、细胞信号传导和细胞增殖。细胞GSH浓度的降低是响应许多细胞中不同凋亡刺激的细胞死亡进展的早期标志。我们的细胞计™细胞内GSH检测试剂盒使用我们专有的ThiolTrace™ Violet 500,在与硫醇(包括细胞中的GSH)反应时会发出强烈的荧光。在正常细胞中,探针具有高度荧光,而在凋亡细胞中,染色强度降低。用ThiolTrace™ Violet染色的细胞可以用流式细胞仪(太平洋橙色滤光片组)观察。该试剂盒可与其他试剂(如 7-AAD (#17501) )一起使用,用于细胞活力和凋亡的多参数研究。该试剂盒针对使用流式细胞仪筛选细胞凋亡激活剂和抑制剂进行了优化。
组件
组分 A:ThiolTrace™ Violet 500 1瓶
组分B:检测缓冲液 1 瓶 (100 mL)
组件C:DMSO 1 瓶 (200 μL)
示例协议
一目了然
协议摘要
- 用密度为 5 × 10 的测试化合物制备细胞5至1×106细胞/毫升
- 准备并添加ThiolTracTM紫色500细胞工作溶液
- 37oC孵化20 到 30 分钟
- 读取Ex/Em = 405/525 nm-太平洋橙色滤光片组时的荧光强度
重要注意事项
在开始实验之前在室温下解冻。
储备液的制备
除非另有说明,否则所有未使用的储备溶液应分成一次性等分试样,并在制备后储存在-20°C。避免反复冻融循环。
ThiolTracTM紫色 500 储备溶液 (500X):
将 200 μL DMSO(组分 C)加入到巯基痕量小瓶中ThiolTracTM紫罗兰色500,搅拌均匀。注意:等分并存储未使用的ThiolTracTM紫罗兰色 500 储备溶液在 -20oC.避免反复冻融循环。
工作溶液的制备
ThiolTracTM紫罗兰500工作溶液(1X):
加入 2 μL ThiolTracTM紫色 500 储备溶液到 1 mL 检测缓冲液(组分 B)或您选择的缓冲液中,并充分混合。注意: ThiolTracTM紫罗兰500 工作溶液可以在含有血清的细胞培养基中制备。
实验方案样本
- 用测试化合物处理细胞所需的时间。注意:对于贴壁细胞,用0.5mM EDTA轻轻提起细胞以保持细胞完整,并在用ThiolTrace™ Violet工作溶液孵育之前用含血清的培养基洗涤细胞一次。注意:适当的孵育时间取决于所使用的单个细胞类型和细胞浓度。优化每个实验的孵育时间。
- 以 1000 rpm 离心细胞 4 分钟,然后在您选择的 1 mL 缓冲液中洗涤细胞(可选)。
- 将细胞重悬于 1 mL ThiolTracTM紫罗兰500工作溶液并在37时孵育oC 培养箱 20 至 30 分钟。
- 使用太平洋橙色滤光片组(Ex / Em = 405 / 525 nm)的流式细胞仪监测荧光强度。对感兴趣的细胞进行门控,不包括碎片。
以下是AAT Bioquest品牌部分产品订购信息,如需其他产品请联系本店客服
| 品牌 | 货号 | 产品名称 | 单位 |
| AAT Bioquest | 22622 | Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence* | 200 Tests |
| AAT Bioquest | 22623 | Cell Explorer™ Live Cell Tracking Kit *Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22624 | Cell Explorer™ Live Cell Tracking Kit *Deep Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22635 | Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence* | 100 Tests |
| AAT Bioquest | 22636 | Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence* | 100 Tests |
| AAT Bioquest | 22640 | Cell Navigator®CDy6 Mitosis Imaging Kit | 100 Tests |
| AAT Bioquest | 22656 | Cell Navigator® Lysosome Staining Kit *Green Fluorescence* | 500 Tests |
| AAT Bioquest | 22670 | Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence* | 500 Assays |
| AAT Bioquest | 22674 | MitoLite™ Blue FX490 | 500 Tests |
| AAT Bioquest | 22675 | MitoLite™ Green EX488 | 500 Tests |
| AAT Bioquest | 22676 | MitoLite™ Orange FX570 | 500 Tests |
| AAT Bioquest | 22677 | MitoLite™ Red FX600 | 500 Tests |
| AAT Bioquest | 22789 | Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence* | 200 Tests |
| AAT Bioquest | 22800 | Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays* | 500 Tests |
| AAT Bioquest | 22801 | Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* | 100 Tests |
| AAT Bioquest | 22802 | Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22804 | Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22809 | Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation* | 100 Tests |
| AAT Bioquest | 22823 | Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22824 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22828 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* | 100 Tests |
| AAT Bioquest | 22829 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* | 100 Tests |
| AAT Bioquest | 22837 | Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22838 | Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22839 | Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22840 | Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors* | 100 Tests |
| AAT Bioquest | 22841 | Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22842 | Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100 Tests |
| AAT Bioquest | 22860 | Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100 tests |
| AAT Bioquest | 22900 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence* | 200 Tests |
| AAT Bioquest | 22901 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22903 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluorescence* | 200 Tests |
| AAT Bioquest | 22904 | Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry* | 100 Tests |
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