IGFBP7 Protein, Human, Recombinant (K95R, His Tag), HPLC-verified
库存:
大量
供应商:
北京义翘神州科技股份有限公司
规格:
50µg
规格/价格 50 µg:3220
1 mg:29480
IGFBP7 Protein, Human, Recombinant (K95R, His Tag), HPLC-verified: 产品信息
纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
生物活性:Immobilized Recombinant Human IGFBP7 Protein (K95R, His Tag) (Cat:13100-H07H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD93 / C1QR1 Protein (Fc Tag) (Cat:12589-H02H) with a linear range of 0.7-5.0 μg/mL.
蛋白构建:A DNA sequence encoding the Human IGFBP7 (Q16270) (Asp30-Leu282) was expressed, with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
种属:Human
预测 N 端:His
分子量:The recombinant Human IGFBP7 consists of 263 amino acids and predicts a molecular mass of 27.59 kDa. It migrates as an approximately 34.02 kDa and 25.18 kDa band in SDS-PAGE under reducing conditions.
缓冲液:
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
IGFBP7 背景信息 Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.
Oh Y, et al. (1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5.
Wilson EM, et al. (1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3.
Lin J, et al. (2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.