- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1012
- 2026年04月22日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Human renal tubular epithelial cell line
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
肾
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
上皮型
- 是否是肿瘤细胞：
否
- 器官来源：
肾
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化人肾小管上皮细胞简介：
产品描述：肾小管上皮细胞对肾功能的发挥起着重要作用。它们几乎能重吸收肾小球滤过液中所有的葡萄糖和氨基酸，并使其它非营养物质排泄到尿液中。肾小管上皮细胞能产生包括细胞因子和趋化因子在内的炎性介质，并通过产生IL-8从而影响和指导白细胞的分化来积极参与急性炎症过程。在肾脏移植后或新月体肾炎的炎症过程中，肾小管上皮细胞表达IL-2 α受体和II类MHC抗原，这表明它们参与肾脏免疫系统损伤的发病机理。
产品货号：IH1012
产品类型: 原代细胞建立的永生化
传代能力: 30代左右
产品形态: 上皮型
培养基：永生化人肾小管上皮细胞专用完全培养基，产品编号：IH1012-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：1周左右货期
CK18抗体免疫荧光染色鉴定
Regulatory Effect of Rhein and Emodin on Proliferation of Human Renal Tubular Epithelial Cell Line and Activity of TGF-β_1gene Promotor
Objective: To observe the effect of rhein and emodin on the proliferation of human renal tubular epithelial cell line( HK-2) and the activities of TGF-β1gene promoter. Methods: Effects of rhein and emodin on proliferation of HK-2 were analyzed by MTT; different lengths of human TGF-β1gene and CAT as reporter gene were constructed and were transferred into HK-2 RMC by transfection reagent. The effects of rhein and emodin( 1 μg / ml,10 μg / ml,50 μg / ml and 100 μg / ml) on the plasmid were determined by CAT-ELISA. Results: Rhein and emodin of different concentrations had regulatory effect on the proliferation of HK-2( P 0. 01),and with dose compliance; rhein and emodin at 1 μg / ml and 10 μg / ml didn't inhibit the activities of phTGF 2. 14. Rhein and emodin at 50 μg / ml markedly inhibited the activities of ph TGF 2. 14,and the inhibition ratio was 38. 0% and 36. 0%,was different from the control group( P 0. 01). Conclusion: Rhein and emodin have regulatory effect on the proliferation of HK-2. Rhein and emodin at 50 μg / ml have regulatory effect on the activity of TGF-β1g
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
缺血再灌注损伤（ischemia/reperfusion Injury，IRI）中，细胞衰老的作用具有双重调节功能，调节衰老细胞可能是改善供体肾脏结果的潜在治疗靶点。人脐带间充质干细胞衍生的外泌体（HUMSC-EVs）在免疫调节和组织微环境稳态中起重要作用，能够通过减少氧化损伤、炎症细胞因子表达、抑制细胞凋亡等机制改善肾脏 IRI 的预后。本文旨在研究 HUMSC-EVs 通过抑制肾小管上皮细胞衰老来实现保护肾脏 IRI 的机制，并探讨了 HUMSC-EVs 与达沙替尼（dasatinib）和槲皮素（quercetin）联合
应该是肾小球内（脏）层上皮细胞，这株细胞目前只有私人收藏，全球细胞库没有严格意义上的足细胞可供出售。 在文献中提到了“人肾小球系膜细胞系（human mesangial cell line,HMCL)由英国伦敦皇家公立医院肾脏中心阮雄中博士惠赠-----HMCL 为用T2SV40 及H2ras 原癌基因转染的永生系,保持正常人肾系膜细胞基本的形态学和生物学特征"。 因为足细胞很难找到，国内一些研究者已改用肾小管上皮细胞hk-2
腺病毒可以在293A细胞中进行转录，表达并可进行包装复制出大量高滴度病毒。293T是一种逆转录包装表达细胞，我做过也可进行表达，但还没有具体鉴定表达后的病毒是否可用。293细胞系是原代人胚肾细胞转染5型腺病毒（Ad 5）DNA的永生化细胞，表达转染的腺病毒5的基因。细胞来源都是人肧肾上皮细胞，293T细胞表达E1A蛋白，SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。293FT细胞能制造高滴度的慢病毒。293A细胞来源于人肾纤维母细胞，组成性表达E1A 和E1B 蛋白
技术资料
