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| PIPES | sigma | V900300-25G | 321.52 | Biochemistry | 生化试剂 | 0 | NDG | STD | 306.22 | 缓冲溶液 | reagent grade | 99% | PIPES dipotassium salt | 108321-27-3 | 499 | BioChem Core | C35 Biochemistry | LR2 | Life Science Chemistry |
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文献和实验Crystallization of Kinesin Family Motor Proteins
) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w
Angiotensin Protein Kinase Assay
in 12 microliters kinase buffer (40 mM PIPES, pH 7.0, 10 mM MnCl2) at 4°C. 2. The kinase reaction is carried out at room temperature in a total volume of 20 microliters that contains: 15 microCi gamma-32P-ATP 5 microliters
1 for details) 55 mM MnCl2 ・4H2 O 15 mM CaCl2 ・2H2 O 250 mM KCl 10 mM PIPES (0.5M, pH 6.7) Chilled to 0ºC before use. Nucleic Acids And Oligonucleotides Plasmid DNA (recombinant plasmid
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