SARS-CoV-2 Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Recombinant Protein(产品说明)
蛋白名称:SARS-CoV-2 Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His Recombinant Protein
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE.
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat:40589-V08B6), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat:40589-V08B6) is 200-800 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 16
蛋白分子量:The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His tag) consists of 1206 amino acids and predicts a molecular mass of 134.1 kDa.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Met1-Pro1213(del(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Amanat, F; et al. Vaccination with SARS-CoV-2 variants of concern protects mice from challenge with wild-type virus. PLoS biology, Pubmed: 34914685
2, Hu, Q; et al. Chimeric mRNA based COVID-19 vaccine induces protective immunity against Omicron and Delta variants. Molecular Therapy - Nucleic Acids