Immunofluorescence staining of fixed HeLa cells with anti-H3K27me3 antibody BT164. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of BT164 (orb1671571) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671571- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western Blot using anti-H3K27me3 antibody BT164 (Nuclear lysate of HeLa(A) (0.0003 ug/ml)- Jurkat(B) (0.0003 ug/ml) and K562(C) (0.01 ug/ml) (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of BT164 (orb1671571) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the anti-H3K27me3 antibody BT164 HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0