Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of In-1 (orb1671845) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671845- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
Western Blot using anti-CD74 antibody In-1. Mouse spleen (A) and mouse lymph node (B) lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of In-1 (orb1671845) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kDa- and this protein is glycosylated at several residues. orb1671845 successfully detected CD74 in mouse spleen and mouse lymph node lysates.