Immunofluorescence staining of fixed MCF7 cells with anti-GM2 ganglioside antibody L55-81. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of L55-81 (orb1671870) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing diffuse membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671870- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.