Flow-cytometry using anti-CD19 antibody 4G7. Human lymphocytes were stained with an isotype control (3.0 - panel A) or the rmeric version of 4G7 (orb1672130 - panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FACSCanto flow-cytometer.