Flow-cytometry using anti-CD3 epsilon (2C11 scFv) and TRP1 (TA99) antibodies. Mouse splenocytes (A)- B16F10 murine melanoma cells (B)- KPC3 pacreas carcinoma cells (C) and KPC3 cells transfected with the Trp1 gene (D) were fixed using 2% PFA- permeusing 0.5% Triton and were subject to a primary treatment of either buffer- mouse-IgG1 chimeric 2C11 or mouse-IgG1 chimeric TA99 (indicated ts) before a secondary treatment with buffer- goat anti-mouse Ig-allophycocyanin (G-aM Ig-APC) or anti-HisTag-APC (aHis-APC) antibodies (indicated beside plots). In panel A- splenocytes were also stained with a commercially avail-CD3 (2C11) antibody conjugated to phycoerythrin (PE)