ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5 ug/ml of Multitag protein (Pr00106- Antibody). Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse (ec- 0300-0108P).