Left Panel: orb1672344 (2 ug/ml) staining of HEK293 spiked (A) and unspiked (B) with 5 ng multitag protein (35 ug protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: .0 (1 ug/ml) staining of Multitag protein at 25 ng (A) - 10 ng (B) and 5 ng (C) amounts. For both - primary incubation was 1 hour. Detected by chemiluminescence.
ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5 ug/ml of Multitag protein (Pr00106-Antibody). Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse (ec-0300-0108P).