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- ACROBiosystems
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- CHEK-ATP087
- 2025年07月30日
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文献和实验Human Pregnane X Receptor (hPXR) Activation Assay in Stable Cell Lines
for the application of screening for human PXR activation using commercially available stable cell lines harboring the PXR and a luciferase reporter gene linked to the promoters of the human CYP3A gene. These instructions provide detailed information on how to thaw
Generation of a Stable Cell Line for Constitutive miRNA Expression
Here, a protocol is described to generate stable cell lines for constitutive expression of single cellular or viral miRNA precursors in absence of infection. The procedure comprises cloning of the precursor sequence, generation of the lentiviral expression vector
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
by a small barrel shaped structure (13 KDa, 2.5 nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2 M NaCl or 0.5% SDS). The GFP-binding protein can be produced in bacteria and purified as a stable monomer. From detailed
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