Hmec-1; HMEC1; CDC/EU.HMEC-1; Human Microvascular Endothelial Cell line-1
种属
人
生长特性
贴壁细胞
细胞形态
上皮细胞样
生长培养基
Complete Growth Medium for HMEC-1
冻存条件
冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件
气相:空气,95%;CO2,5%温度:37℃
推荐传代比例
1:3-1:5
推荐换液频率
2~3次/周
背景描述
Microvascular endothelial cells isolated from human foreskins were transfected with pSVT vector, a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen.