手机验证
无
NCI-H295R
无
1x10^6 cells
博辉生物科技(广州)有限公司
详询
NCI-H295R
详询
NCI-H295R
人肾上腺皮质腺癌细胞
人肾上腺皮质腺癌细胞
人/Human
未知
贴壁
是
人肾上腺皮质腺癌细胞
干冰/常温
不限
上皮细胞样
冻存管/培养瓶
目录号 | BH-C371 |
细胞英文(简称) | NCI-H295R |
细胞名称 | NCI-H295R; 人肾上腺皮质腺癌细胞 【已通过STR鉴定】 |
背景资料 | 该细胞系源于多能肾上腺皮质癌细胞系NCI-H295,后者由Gazdar AF等建立,从肾上腺皮质的肿瘤中分离而来。NCI-H295细胞经改变培养条件获得了NCI-295R细胞,群体倍增时间从原来的5天减为2天。NCI-295细胞悬浮生长,而NCI-H295R呈单层贴壁生长。NCI-H295R细胞保留了产生雄激素的能力,对血管紧张素Ⅱ和钾离子有反应。 |
细胞来源 | ATCC |
代次 | P3 |
规格 | 冻存管/培养瓶 |
细胞数 | 1x10^6 cells |
生物安全级别 | 2 |
组织来源 | 肾 |
细胞形态 | 贴壁;上皮细胞样 |
细胞活力 | 95%(Viability by Trypan Blue Exclusion) |
细胞检测 | 细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌 |
培养条件 | DMEM/F12 +10% FBS+1%ITS-G+1%P/S;37℃,5% CO2 |
传代方法 | 建议1:2-1:3 两天换液一次 |
冻存条件 | 无血清细胞冻存液(货号:S002) |
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资料下载:
NCI-H295R(BH-C371).docx 附件下载 (下载 0 次)
A5培养操作说明.pdf 附件下载 (下载 0 次)
A5细胞收到处理方法.pdf 附件下载 (下载 0 次)
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