- ￥7000
- GeneTex
- 美国
- GTX34569
- 2025年07月12日
- WB, ICC/IF, IHC-P
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human full-length CD86 protein
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX34569
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
CD86
- 抗体英文名：
CD86 antibody [C86/1146]
- 抗体名：
CD86 抗体 [C86/1146]
- 规格：
100 μg
ICC/IF analysis of Raji cells using GTX34569 CD86 antibody [C86/1146].
Green : Primary antibody
Red : nucleus
WB analysis of Daudi cell lysate using GTX34569 CD86 antibody [C86/1146].
IHC-P analysis of human melanoma tissue using GTX34569 CD86 antibody [C86/1146].
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文献和实验Yunke Liu et al., J Healthc Eng 2022 (PMID:35432831)
Fang SY et al., Exp Ther Med 2021 (PMID:33732342)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
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