- ￥5300
- GeneTex
- 美国
- GTX85702
- 2025年07月11日
- WB, ICC/IF, IHC-P, ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide - KLH conjugated
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX85702
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
- 抗体英文名：
HIF3 alpha antibody
- 抗体名：
HIF3 alpha 抗体
- 规格：
50 μg
Human Placenta (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Anti-Hif Antibody - Western Blot. Western blot of Affinity Purified anti-Hif antibody shows detection of a band ~72 kD corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37 degree C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:1600. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with HIF3A antibody (GTX85702) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
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文献和实验Sun DG et al., Front Endocrinol (Lausanne) 2020 (PMID:32296392)
Qu X et al., Anal Cell Pathol (Amst) 2022 (PMID:35915851)
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