The Quick-Neuron™ Cholinergic - mRNA Kit facilitates rapid and efficient differentiation of human iPS or ES cells into cholinergic neurons in just 7 days. Our proprietary transcription factor-based stem cell differentiation method uses synthetic mRNAs to produce highly pure populations of neurons without a genetic footprint. Quick-Neuron™ Cholinergic differentiated cell cultures display typical neurite outgrowth and express a variety of neuronal markers, such as TUBB3 (pan-neuronal) and ChAT (cholinergic).
High-Throughput Screening Assay for Detecting Drug-Induced Changes in Synchronized Neuronal Oscillations and Potential Seizure Risk Based on Ca2+ Fluorescence Measurements in Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neuronal 2D and 3D Cultures. Cells. 2023 Mar 21;12(6):958.Efficient derivation and banking of clinical-grade human embryonic stem cell lines in accordance with Japanese regulations. Regen Ther. 2022 Nov 6;21:553-559.Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation. Commun Biol. 2023 Mar 30;6(1):347.Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis. STAR Protoc. 2022 Apr 28;3(2):101360.Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons. iScience. 2023 Feb 27;26(4):106285.