Epigenase m6A甲基化酶活性/抑制分析试剂盒(比色法) 产品名称:Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)
货号:P-9019
产品描述:This kit is designed for measuring total m6A methylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A methylases bind to and methylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。 产品特点: 1·Fast: Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours; 2·Robust: Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent; 3.Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro. 4.Sensitive and Specific: Novel assay principle allows to detect the m6A methylase-converted end product, which makes the assay much more specific than that for by-product measurement. The assay also allows high sensitivity to be achieved. The activity can be detected from as low as 2 µg of nuclear extracts or 50 ng of purified enzymes; 5.Quantitative: The assay standard is included, which allows the specific activity of m6A methylase to be quantified; 6.Flexible: Strip-well microplate format makes the assay flexible for manual or high throughput analysis。