Silver sulfadiazine磺胺嘧啶银,22199

Silver Enhancement Protocol

for microbial contamination before each use if not making fresh each time. 2. Solution A: Dissolve 100 mg silver acetate (Fluka Chemical Co., catalog # 85140) in 50 ml distilled water and keep under cover. To speed dissolving the fluffy powder, stir

Silver: Colony PCR

  Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots at -20°C (minimize freeze-thawing if possible) Make 2.5 mg/ml zymolyase

Silver: Lysate for Western

  Grow cells Harvest Spin down Wash with PBS Spin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tube Prepare lysis buffer (beforehand) and chill on ice PBSMT