The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-5/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for excitation 490 nm/emission 510 nm (FITC) for 490-reagent detection, excitation 550 nm/emission 570 nm (Cy3) for 550-reagent detection, and excitation 640 nm/ emission 660 nm (Cy5) for 640-reagent detection. Filters should have high specificity to avoid bleed-through artifacts.
产品名:FluoroSpot Plus: Human IFN-gamma/IL-5/TNF-alpha
货号:orb1532850
产品中文名:FluoroSpot Plus: Human IFN-gamma/IL-5/TNF-alpha试剂盒
产品详情链接:https://www.biorbyt.com.cn/fluorospot-plus-human-ifn-gam-orb1532850.html 武汉博欧特生物科技有限公司(www.biorbyt.com.cn )为英国品牌Biorbyt(www.biorbyt.com )中国区总代理。负责biorbyt品牌产品在中国市场的推广、销售及售后服务。为您提供各种biorbyt原装进口产品,主推四大类: