CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; FLJ41520; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17; RAD17 homolog (S. pombe); RAD17 homolog; Rad17 like protein; RAD17, S. pombe, homolog of; RAD17_HUMAN; RAD17Sp; Rad24; Rad24, mouse, homolog of; Rad24, S. cerevisiae, homolog of; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog
背景资料
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Swiss Prot
O75943
Gene ID
5884
亚细胞定位
Nucleus
宿主
Rabbit
克隆类型
Polyclonal Antibody
亚型
IgG
预测交叉反应
Monkey;Orangutan
蛋白分子量
57/66/76/77kDa
实际分子量
72kDa
稀释比例
WB 1:1000-3000.
免疫原
Recombinant protein of human RAD17
Public Immunogen Range
1-350aa
纯化方法
Affinity purification
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存
Store at -20℃. Avoid freeze / thaw cycles.
交叉反应
Human;Mouse
应用
WB
规格
50ul 100ul
单位
支
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示:
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
"Western blot analysis with RAD17 antibody diluted at 1:2000;Lane: U937