凯杰Qiagen218161逆转录试剂盒miScript I

AllPrep DNA/RNA 96 Kit

using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates

基因表达之RT-PCR之我见

对大家有启发，在这里说一下，实际上主要是温度，至于放在TRIZOL中是不可取的，更不要说在胍里了，只－20是不够的，至少－80，液氮最保险，想想，在低温里，即使加上些RNA酶它也降解不了哇！qiagen出了一种easy产品可用于保存标本，但在常温也只是1天，所以低温才是首要的，抽的时候也是这样。3、反转录：常规，取样尽量一致，如上所述，不是为了定量，是为了半定量PCR时图形的好看。选择逆转录酶根据实验需要，少量的可用Promega的一种1ug的酶，多的我们用invitrgen的5ug的II，当然有人

Dynabeads® mRNA DIRECT Kit

by reverse transcriptase (RT)-PCR from highly specialized cells (e.g. isolated from a heterogenous sample by immunomagnetic separation). In addition, the protocol has been successfully used to isolate mRNA from a wide variety of tissues of mammalian, fish