手机验证
8 Rxns、48 Rxns
NEXTflex™ Rapid Directional RNA-Seq Kit
>1年
蓝景科信
-20℃/2~8℃
NEXTflex™ Rapid Directional RNA-Seq Kit 为mRNA 或去除rRNA 总RNA的单端、双端和多重链特异性文库构建提供一个简单、灵活的解决方案。这个试剂盒可以用于获取RNA转录中DNA模板链的信息,准确率大于99%。与普通转录组测序相比,它更能准确地统计转录本的数量和确定基因的结构,同时可以发现更多的反义转录本,目前被广泛地应用于研究基因结构和基因表达调控等领域范围,并能够减少每个样本的测序费用。
NEXTflex™ Rapid Directional mRNA-Seq Kit 套装
为了简化mRNA 测序,PerkinElmer公司现在提供一个套装,包括NEXTflex Rapid Directional RNA-Seq library 建库试剂,NEXTflex RNA-Seq Barcodes 和NEXTflex Poly(A) Beads 。这个试剂盒有两个不同的版。每个试剂盒包含48次的NEXTflex Rapid Directional RNA-Seq Kit,NEXTflex Poly(A) Beads (48 rxns )和24种独立NEXTflex RNA-Seq Barcodes。
产品特点:
多重测序
NEXTflex Rapid Directional RNA-Seq Kit与NEXTflex™ RNA-Seq Barcodes 或者NEXTflex-96™ RNA-Seq Barcodes一起用于多重测序。这个多达96种独立接头的高通量试剂盒适用于Illumina RNA 测序平台。
产品列表:
货号 | 产品名称 | 规格 |
NOVA-5138-07 | NEXTflex™ Rapid Directional RNA-Seq Kit |
8 RXNS |
NOVA-5138-08 | NEXTflex™ Rapid Directional RNA-Seq Kit |
48 RXNS |
NOVA-5138-10 | NEXTflex™ W/ RNA-SEQ BARCODES 1 - 24 & POLY(A) BEADS |
48 RXNS |
NOVA-5138-11 | NEXTflex™ W/ RNA-SEQ BARCODES 25 - 48 & POLY(A) BEADS | 48 RXNS |
Figure 1.与竞争者N 文库产量的比较
1) 插入片段范围为200-400bp(Agilent Bioanalyzer)(Figure 5A)
2) Figure 1B 代表Comp N的文库量,Figure 5C 代表NEXTflex 1:5 稀释的文库量,NEXTflex的文库浓度是Competitor N的四倍
rRNA and Other Non-Coding RNA
Figure 2. rRNA Mapping
RNA建库过程有少量的rRNA污染,但是NEXTflex Rapid Directional RNA-Seq Kit与Competitor N’s kit相比,rRNA 的读取率低,文库量高,有用的测序reads所占比例高
Read Mapping
Table 1. Unique Mapping Rate and Number of Unique Reads.
Kit Name | Unique Mapped | Unique Mapped Reads (millions) |
NEXTflex™ Rapid Directional RNA-Seq Kit | 53.49% ± 1.16% | 1.604 ± 35 |
Competitor N Directional RNA-Seq Kit | 44.08% ± 3.95% | 1.321 ± 118 |
Figure 3. Reads Mapping Metrics
NEXTflex Rapid Directional RNA-Seq Kit比较结果:
1) 更高的唯一mapped reads百分比
2) 较低的重复率
3) 检测出更多的转录本
KIT CONTENTS
REQUIRED MATERIALS NOT PROVIDED
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