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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fragmemt of human alpha Tubulin.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX638140
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha Tubulin
- 抗体英文名:
alpha Tubulin antibody [HL2162]
- 抗体名:
alpha Tubulin 抗体 [HL2162]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Tubulin antibody [HL2162] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
alpha Tubulin antibody [HL2162] detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Tubulin antibody [HL2162] detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
alpha Tubulin stained by alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [HL2162] (GTX638140) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
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