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| Bioland™ 酶标板;透明;高结合力;F型底;无 RNase/DNase,无热原 | 贝兰伯 | PT06-96S | 58.00 | 10个/包;100个/箱 | IVD诊断 | Bioland | 包 |
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文献和实验to avidin magnetic beads (Dynal™). The final elutes, following the binding of the biotin linked RNAs with the avidin magnetic beads and washing, were then DNAse, RNAse H or RNAse A treated, RT (BioRad™ iScript) reacted and PCR amplified. References 1.Kim
Avoiding DNA Contamination in RT-PCR
for routine use in systems in which genomic DNA contamination is a problem. The use of high quality, RNase-free DNase is crucial. Two additional conventional methods of reducing contaminating genomic DNA from total RNA preparations are acid phenol extraction
empty, centrifuge the Midi column for 10 min at >4000 x g to completely dry the HiBind™ matrix. 9. Elution of RNA. Transfer the column to a new RNase-free 15 ml centrifuge tube (not supplied with kit) and elute the RNA with 0.3-0.5 ml of DEPC water (supplied
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