英文名称 Recombinant CDV Fusion Glycoprotein F0 protein, His 中文名称 重组犬瘟热病毒融合糖蛋白F0蛋白 英文别名 Canine Distemper Virus Fusion Glycoprotein F0; Fusion glycoprotein F0 · Canine distemper virus (strain Onderstepoort) (CDV); FUS_CDVO; Recombinant CDV Fusion Glycoprotein F0 protein, His 分子量 52 浓度 >0.5 mg/ml 储存液 10 mM Tris-HCl (pH=8.0) with 8 M Urea and 10% Glycerol. 保存条件 Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
重组犬瘟热病毒融合糖蛋白F0蛋白
标签 His 纯度 >95% as determined by SDS-PAGE 内毒素 Not analyzed 重组蛋白-表达系统 E.coli
背景资料:Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (F protein) probably interacts with H at the virion surface. Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes. Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis