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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃
- 英文名:
E. coli RecQ DNA helicase, functional
- 供应商:
上海壹科繁生物科技有限公司
- 规格:
100 ug/20 ug
| 规格: | 100 ug | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 20 ug | 产品价格: | 询价 |
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文献和实验Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
of active polymerase mutants, for screening of polymerase inhibitors, or for screening of mutants with altered properties. Here we describe complementation of JS200 cells with the wild-type E. coli DNA polymerase I to illustrate such functional polymerase
Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
Preparation of Chromosomal DNA from E. coli
This chapter describes a simple and rapid way of extracting and purifying chromosomal DNA from E. coli and many other species of bacteria. This procedure is essentially a simplified version of that described by Marmur in 1961 (1 ). The cells
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