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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海壹科繁生物科技有限公司
- 规格:
10set
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文献和实验E. Z. N.A.TM X-press Plasmid Protocol
1.5 and 2.0 mL Centrifuge Tubes 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate selective antibiotic. Incubate 14-16 hours at 37℃
Basic procedures for bacteria culture
(EtBr), swirl to mix, and pour the gel onto a taped plate with casting combs in place. Allow 20-30 minutes for solidification. 3. Carefully remove the tape and the gel casting combs and place the gel in a horizontal electrophoresis apparatus. Add
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Liquid nitrogen storage tank Microscope Pipettes (5- or 10-mL) or cell scraper (see Step 15) Plates (tissue culture, six-well) METHOD Preparation of Conditioned Medium (CM) 1. Plate irradiated MEFs at 20,000
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