Capsid protein VP2; Coat protein VP2; Canine parvovirus; CAPSD_PAVC; Capsid protein VP2 · Canine parvovirus type 2 (CPV-2); Recombinant CPV2 VP2 protein, His
理论分子量
42kDa
性 状
Lyophilized or Liquid
浓 度
>0.5 mg/ml
物 种
CPV
序 列
201-584/584
纯 度
>95% as determined by SDS-PAGE
纯化方法
AC
内毒素
Not analyzed
表达系统
E.coli
活性
Not tested
标签
His
缓 冲 液
0 mM Tris-HCl (pH=8.0) with 150 mM NaCl.
保存条件
Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
重组犬细小病毒VP2蛋白
产品介绍:Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids.