大鼠Fractalkine AccuSignal ELISA试剂盒-KOA 0176,Rat Fractalkine AccuSignal ELISA Kit - KOA0176,大鼠Fractalkine AccuSignal ELISA试剂盒-KOA 0176,Rat Fractalkine AccuSignal ELISA Kit - KOA0176
产品名称:大鼠Fractalkine AccuSignal ELISA试剂盒-KOA 0176-Rat Fractalkine AccuSignal ELISA Kit - KOA0176
产品货号:KOA0176
产品规格:1Kit
背景资料:Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.
保存建议:大鼠Fractalkine AccuSignal ELISA试剂盒-KOA 0176-Rat Fractalkine AccuSignal ELISA Kit - KOA0176Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
产品描述:Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml rat Fractalkine standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. It is recommended that each rat Fractalkine standard solution and each sample be measured in duplicate.
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