大鼠纤连蛋白AccuSignal ELISA试剂盒-KOA 0170,Rat Fibronectin AccuSignal ELISA Kit - KOA0170,大鼠纤连蛋白AccuSignal ELISA试剂盒-KOA 0170,Rat Fibronectin AccuSignal ELISA Kit - KOA0170
产品名称:大鼠纤连蛋白AccuSignal ELISA试剂盒-KOA 0170-Rat Fibronectin AccuSignal ELISA Kit - KOA0170
产品货号:KOA0170
产品规格:1Kit
背景资料:Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250KDa.
保存建议:大鼠纤连蛋白AccuSignal ELISA试剂盒-KOA 0170-Rat Fibronectin AccuSignal ELISA Kit - KOA0170Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
产品描述:Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml rat Fibronectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each rat Fibronectin standard solution and each sample is measured in duplicate.
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