Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG®) Chemiluminescent Western Blotting Kit - KCA383
库存:
100
供应商:
艾美捷科技
CAS号:
参考产品说明书
规格:
1Kit
化学发光免疫印迹试剂盒:DYKDDDDK(FLAG®)化学发光蛋白质印迹试剂盒-KCA 383,Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG®) Chemiluminescent Western Blotting Kit - KCA383,化学发光免疫印迹试剂盒:DYKDDDDK(FLAG®)化学发光蛋白质印迹试剂盒-KCA 383,Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG®) Chemiluminescent Western Blotting Kit - KCA383
背景资料:The FLAG® epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG® -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG®) antibodies. The anti-DYKDDDDK (FLAG®) antibody included in this Western blot kit detects FLAG® -tagged recombinant proteins fused with FLAG® tag either at the amino-terminus or carboxy-terminus of the protein.
产品描述:Use Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western Blotting for detection of FLAG® -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG®). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.