转录因子靶向ChIP-seq试剂盒-KOA 0882,Transcription Factor Targeted ChIP-seq Kit - KOA0882,转录因子靶向ChIP-seq试剂盒-KOA 0882,Transcription Factor Targeted ChIP-seq Kit - KOA0882
产品名称:转录因子靶向ChIP-seq试剂盒-KOA 0882-Transcription Factor Targeted ChIP-seq Kit - KOA0882
产品货号:KOA0882
产品规格:1Kit
背景资料:Association between proteins and DNA is a major mechanism in many vital cellular functions such as gene transcription and epigenetic silencing. It is crucial to understand these interactions and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is a technique to analyze the association of proteins with specific genomic regions in intact cells. ChIP can be used to study changes in epigenetic signatures, chromatin remodelling and transcription regulator recruitment to specific genomic sites.In ChIP, living cells are first fixed with a reversible cross-linking agent to stabilize protein-DNA interactions. The most widely used reagent to fix cells is formaldehyde which generates covalent bonds between amino or imino groups of proteins and nucleic acids. Formaldehyde treatment crosslinks both DNA-protein as well as protein-protein complexes.Following cross-linking, chromatin needs to be sheared very efficiently into homogeneous small fragments that can subsequently be used in immunoprecipitation (IP). After fragmentation, the sheared chromatin is precipitated with a specific antibody (AB) directed against the protein of interest. The chromatin-AB complex is isolated using magnetic beads. Finally, the precipitated DNA fragments are released from the AB, and analyzed. Enrichment of specific sequences in the precipitated (IP’d) DNA indicates that these sequences were associated with the protein of interest in vivo. Analyzis of specific regions can be performed by quantitative polymerase chain reaction (qPCR). In recent years, ChIP combined with high-throughput Next-Generation sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions.
保存建议:转录因子靶向ChIP-seq试剂盒-KOA 0882-Transcription Factor Targeted ChIP-seq Kit - KOA0882See kit insert for components stored at 2-8°C and -20°C. Do not freeze Protein A and magnetic beads.
产品描述:The different steps of the ChIP assay are cell fixation (cross-linking), chromatin shearing, immunoprecipitation, reverse cross-linking followed by DNA purification and analysis of the immunoprecipitated DNA. Suitable for 10 reactions.
点击:转录因子靶向ChIP-seq试剂盒-KOA 0882-Transcription Factor Targeted ChIP-seq Kit - KOA0882查看更详细产品说明,更多应用、储存、价格、货期等信息请致电027-59626688垂询艾美捷科技有限公司。更多Rockland公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
Rockland成立于1962年,是一家老牌抗体原产企业,其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体,包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素(Ubiquitin)等识别抗体,应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外,Rockland也生产特别齐全的二抗,包括纯化、生物素、HRP/ALP、荧光标记二抗,应用十分广泛。
艾美捷科技有限公司,通过提供完整的产品和服务体系、便利的客户关系和供应链管理,为生命科学研究工作者提供整体打包方案。我们的企业的核心价值观:“学习、创新、合作、卓越"。作为一家具有尖端的技术实力、的经营管理水平和完善的市场销售体系的生物高科技企业,总部位于武汉光谷高新技术开发区,服务面向全国。艾美捷科技是集进口试剂、实验室耗材销售、技术服务与合约开发为一体的专业化高科技公司,为用户提供最前沿的专业资讯、完备的产品、整合的解决方案,及优质的物流服务。为了更好的服务客户,公司组建了一支经验丰富的研发团队-艾美捷生物技术中心,进入研发生产阶段,将更优质的产品推荐给国内生物领域的同仁们!