一般描述 IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline® DryStrip gels. 应用 Each IPG Buffer type produces more uniform conductivity along the Immobiline® DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining.
IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip. 包装 1 pkg 特点和优势
For use with Immobiline® DryStrip gels to improve Protein solubility.
Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins.
Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas.